Open AccessAssembly of the Mitochondrial Membrane System: Mutations in the pho2 Locus of the Mitochondrial Genome of Saccharomyces cerevisiae
Author(s) -
CORUZZI Gloria,
TREMBATH M. Kaye,
TZAGOLOFF Alexander
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12746.x
Subject(s) - locus (genetics) , mutant , mitochondrial dna , saccharomyces cerevisiae , gene , genetics , biology , mitochondrion , inner mitochondrial membrane , microbiology and biotechnology , genome
Two mutants of Saccharomyces cerevisiae which show a loss of mitochondrial rutamycin‐sensitive ATPase activity are described. Although phenotypically similar to mutants of the mitochondrial locus pho1 [F. Foury and A. Tzagoloff (1976) Eur. J. Biochem. 68 , 113–119], these mutants define a second ATPase locus on the mitochondrial DNA (designated pho2 ), which is genetically unlinked to pho1 . Analysis of recombination in crosses involving multiple antibiotic resistance markers indicates that the locus is in the segment of the genome between ery1 and oli2 , very close to oli1 . In fact it is proposed that the oli1 and pho2 mutations are in the same gene. Supporting evidence for this proposal includes:1 The analysis of marker retention in petite mutants shows that the oli1 and pho2 loci were either retained or lost together in all cases. 2 Recombination frequencies of 0.05% or less are observed in crosses between the oli1 and pho2 loci. 3 When Q + revertants are isolated from the pho2 mutants they frequently are oligomycin resistant. 4 pho2 mutants have an altered subunit 9 of the ATPase complex.