Open AccessStructure and Replication of Echovirus Type 12
Author(s) -
ROSENWIRTH Brigitte,
EGGERS Hans J.
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12722.x
Subject(s) - echovirus , replication (statistics) , virology , type (biology) , biology , computer science , enterovirus , virus , paleontology
The propagation of echovirus 12 in green monkey kidney (GMK) cells, a continuous cell line, is described. Virions and empty capsids are purified to homogeneity by CsCl density and by velocity centrifugation. The protein composition of the particles as compared to poliovirus 2 particles is analyzed by dodecyl sulfate/polyacrylamide gel electrophoresis. Infectious virions were found to be composed of four different polypeptide chains of molecular weights 33 000, 31 000, 24 000 and 7000. Some additional polypeptide bands at low concentrations are also visible. The empty capsids lack the 31 000‐ M r and 7000‐ M r components and contain instead a polypeptide of the molecular weight 46 000. The molecular weights of the structural polypeptides are the same regardless of whether the virus has been synthesized in primary African green monkey kidney cells or in the continuous GMK cell line. The sedimentation behavior of echovirus 12 RNA is shown to be similar to that of poliovirus 2 RNA.