Open AccessPhotooxidation of Soybean Apoleghemoglobin with Protoporphyrin IX, Heme and Dye
Author(s) -
PERTTILÄ Ulla,
ELLFOLK Nils
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12684.x
Subject(s) - histidine , methylene blue , protoporphyrin ix , rose bengal , heme , chemistry , photochemistry , tryptophan , irradiation , protoporphyrin , porphyrin , methylene , amino acid , biochemistry , photodynamic therapy , medicinal chemistry , organic chemistry , photocatalysis , catalysis , enzyme , physics , nuclear physics
Soybean apoleghemoglobin a was irradiated with visible light in the presence of different sensitizers to probe the heme environment of the protein. With protoporphyrin IX as sensitizer, specific photooxidation of histidine‐92 and histidine‐61 occurred. Irradiation of oxyleghemolglobin and cyanleghemoglobin resulted in photooxidation of histidine‐92, while addition of methylene blue caused both histidine‐92 and histidine‐61 to be oxidized. Apoleghemoglobin, irradiated in the presence of rose bengal or methylene blue, lost tryptophan‐128 in addition to the two histidines.