Open AccessPolynucleotide‐Protein Interactions in the Translation System
Author(s) -
TURCHINSKY Michail F.,
BROUDE Natalia E.,
KUSSOVA Klavdia S.,
BUDOWSKY Edward I.,
ABDURASCHIDOVA Gulnara G.,
MUCHAMEDGANOVA Elene V.,
SCHATSKY Ivan N.,
BYSTROVA Tamara F.
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12577.x
Subject(s) - ribosome , polynucleotide , escherichia coli , translation (biology) , ribosomal rna , rna , ribosomal protein , 30s , translation system , protein biosynthesis , microbiology and biotechnology , chemistry , biology , biochemistry , messenger rna , gene , in vitro
Direct contacts between 16‐S RNA and split proteins S2, S3, S5, S14 and S21 inside the 30‐S subunit of Escherichia coli ribosomes were evidenced by the formation of ultraviolet‐induced (λ= 254 nm) RNA‐protein cross‐links. 30‐S subunits were reassembled from core particles and a mixture of split proteins containing in each case a single 125 I‐labelled protein. All the proteins tested are cross‐linked as a result of a single‐hit process; proteins S3 and S21 were cross‐linked at the highest rate.