Open AccessComparison of the RNA‐Dependent DNA Polymerase of an Endogenous Avian Leukosis Virus to the Polymerase of an Exogenous Avian Leukosis Virus
Author(s) -
BAUER Georg,
SOO Ruth,
FRIIS Robert R.
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12570.x
Subject(s) - polymerase , dna polymerase , biology , enzyme , rous sarcoma virus , rna , microbiology and biotechnology , virology , rna directed dna polymerase , virus , rna polymerase , dna , endogeny , rna dependent rna polymerase , reverse transcriptase , biochemistry , gene
RNA‐dependent DNA polymerases from Rous‐associated virus‐O and avian myeloblastosis virus were partially purified by affinity chromatography and compared to each other. The enzymes are indistinguishable in the immunoglobulin inhibition test and by several enzymological criteria, such as optimum curves for the concentrations of Mg 2+ , K + , H + ; monophasic Lineweaver‐Burk plot for dTTP and biphasic Lineweaver‐Burk plot for dGTP. In thermal inactivation studies a small difference can be observed, suggesting a minor difference in the physical structures of the enzymes. Our findings are consistent with the idea that the RNA‐dependent DNA polymerases of endogenous and exogenous avian leukosis viruses are very closely related to each other and therefore may be regarded as one group of polymerases.