Open AccessMechanism of Spermine Stimulation of Rat‐Liver Isoleucyl‐tRNA Formation
Author(s) -
IGARASHI Kazuei,
EGUCHI Kuniko,
TANAKA Makoto,
HIROSE Seiyu
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12569.x
Subject(s) - spermine , stimulation , mechanism (biology) , transfer rna , chemistry , biophysics , microbiology and biotechnology , endocrinology , biochemistry , biology , rna , physics , gene , enzyme , quantum mechanics
The mechanism of spermine stimulation of rat‐liver isoleucyl‐tRNA formation has been studied by Cleland's method [ Biochim. Biophys. Acta 67 , 104–137 & 173–197 (1963)]. It was demonstrated that isoleucyl‐tRNA formation proceeded through a ping‐pong mechanism in the presence of spermine and magnesium. From a study of the effect of spermine on both the formation of isoleucyl‐AMP · enzyme complex and on the subsequent formation of isoleucyl‐tRNA from the isoleucyl‐AMP · enzyme complex, evidence is presented that the stimulatory effect of spermine functions mainly through the spermine preventing the tRNA inhibition of isoleucyl‐AMP · enzyme complex formation.