Open AccessReaction of tRNA Phe from Yeast with 1‐Fluoro‐2,4‐dinitrobenzene
Author(s) -
WATANABE Kimitsuna,
CRAMER Friedrich
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12545.x
Subject(s) - adenosine , transfer rna , residue (chemistry) , dinitrophenyl , ribose , chemistry , stereochemistry , yeast , biochemistry , rna , enzyme , biology , antibody , genetics , gene
The reaction of 1‐fluro‐2,4‐dinitrobenzene with tRNA Phe from yeast, for the introduction of antigenic‐determining 2,4‐dinitrophenyl residues into tRNA, took place only at adenosine residues in tRNA Phe . After reaction at pH 8.0 and 50 °C two kinds of products were detected: one was ribose‐modified adenosine which was derived from the 3′ terminus of tRNA, and the other was base‐modified adenosine. The sites and extent of the modification of each particular adenosine residue of tRNA Phe were determined as follows: 5(6% modified), 31(2%), 35(36%), 67(51%). and 76(51%). Thus mainly the terminal adenosine and one adenosine in the anticodon loop bear the 2,4‐dinitrophenyl residue.