Open AccessRNA‐Protein Neighbourhoods of the Ribosome Obtained by Crosslinking
Author(s) -
BÄUMERT Hans G.,
SKÖLD SvenErik,
KURLAND Charles G.
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12536.x
Subject(s) - ribosome , ribosomal rna , rna , ribosomal protein , heterologous , nucleic acid , guanine , chemistry , biochemistry , 5.8s ribosomal rna , 50s , microbiology and biotechnology , biology , nucleotide , gene
It is possible to crosslink protein to nucleic acid with diepoxybutane which reacts mainly with the N‐7 of guanine as well as the thiol and aminogroups in protein; the resulting crosslinked complexes are cleavable. When this reagent is applied to ribosomes, even under conditions that minimize the extent of reaction, at least half of the ribosomal proteins can be recovered crosslinked to one or other of the major ribosomal RNA species. In addition, one fourth of the proteins can be recovered crosslinked to the RNA of the heterologous ribosomal subunit. Some of the remaining proteins may also be crosslinkable to the RNA, but additional experiments are required to confirm this.