Open AccessDiminished Sensitivity of Re‐initiation of Translation to Inhibition by Cap Analogues in Reticulocyte Lysates
Author(s) -
ASSELBERGS Fred A. M.,
PETERS Wilbert,
VENROOIJ Walther J.,
BLOEMENDAL Hans
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12473.x
Subject(s) - reticulocyte , nuclease , protein biosynthesis , initiation factor , translation (biology) , globin , messenger rna , lysis , eukaryotic initiation factor , chemistry , eukaryotic translation , biochemistry , microbiology and biotechnology , biology , gene
In a nuclease‐treated reticulocyte lysate reinitiation of protein biosynthesis was less inhibited by 7‐methylguanosine 5′‐monophosphate (m 7 GMP), whereas primary‐initiation could be totally blocked by cap analogues. We suggest that a cap binding factor binds to mRNA as a first step in initiation of protein synthesis and that this factor remains bound during the subsequent reinitiation cycles. Primarily because of the greater relative importance of the reinitiation process, globin synthesis in the untreated lysate was less inhibited by m 7 GMP.