Open AccessStructure of the Hexose Region of Shigella sonnei Phase II Lipopolysaccharide with 3‐Deoxy‐ d ‐ manno ‐octulosonic Acid as Possible Immunodeterminant and Its Relation to Escherichia coli R 1 Core
Author(s) -
KONTROHR Tivadar,
KOCSIS Béla
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12447.x
Subject(s) - galactose , escherichia coli , microbiology and biotechnology , sugar acids , biochemistry , lipopolysaccharide , chemistry , hexose , polysaccharide , glycan , biology , enzyme , sugar , gene , endocrinology , glycoprotein
Comparative chemical analysis (methylation, gas chromatography/mass spectrometry, periodate oxidation, etc.) of the lipopolysaccharides and degraded polysaccharides derived from Shigella sonnei phase I, phase II and galactose‐deficient R mutants revealed a structure as shown:3‐Deoxy‐ d ‐ manno ‐octulosonic acid (dOclA) as an immunodeterminant was observed in the passive hemolysis inhibition test by (a) selective inhibition of the phase II system by dOclA; (b) the kinetics of the change of serological activity during mild acid treatment: 1% acetic acid abolished serological activity; (c) a lack of activity in galactose‐less R mutants and reactivity with R e mutants including Salmonella minnesota R e An enhanced sensitivity of phase II lipopolysaccharide to galactose oxidase after prolonged treatment with 1% acetic acid suggests that dOclA is linked to C‐6 of the terminal or subterminal galactose. dOclA as immunodeterminant could explain some different polysaccharide structures described for Escherichia coli R 1 core.