Open AccessNuclear‐Magnetic‐Resonance Studies of 5′‐Ribonucleotide and 5′‐Deoxyribonucleotide Conformations in Solution Using the Lanthanide Probe Method
Author(s) -
DOBSON Christopher M.,
GERALDES Carlos F. G. C.,
RATCLIFFE George,
WILLIAMS Robert J. P.
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12446.x
Subject(s) - chemistry , protonation , ribonucleotide , deoxyribonucleotides , nucleotide , lanthanide , furanose , nucleobase , guanine , coupling constant , aqueous solution , crystallography , ring (chemistry) , organic chemistry , dna , ion , biochemistry , physics , particle physics , gene
The conformations of the metal‐bound 5′‐ribonucleotides and 5′‐deoxyribonucleotides in aqueous solution at different pH values have been studied using the lanthanide probe method. The conformational analysis, based on mixing different conformations in fast exchange within the nuclear magnetic resonance time scale, agrees well with the results from coupling constants, nuclear Overhauser effects and spin‐lattice relaxation times, obtained for the metal‐fixed systems. The equilibrium between the two basic conformational combinations for the 5′‐nucleotides, anti‐(N⇄S)‐gg‐g'g′ and syn‐(N⇄S)‐gt‐g'g′ depends on the nature of the furanose ring, the base and also on the state of base protonation and phosphate ionization. The effect of base protonation is particularly strong for the guanine nucleotides.