Open AccessMethionyl‐tRNA Synthetase from Sheep Liver
Author(s) -
KELLERMANN Odile,
BREVET Annie,
TONETTI Hubert,
WALLER JeanPierre
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12439.x
Subject(s) - trypsin , biochemistry , size exclusion chromatography , enzyme , transfer rna , sodium dodecyl sulfate , biology , gel electrophoresis , polyacrylamide gel electrophoresis , molecular mass , microbiology and biotechnology , chemistry , rna , gene
The size distribution of methionyl‐tRNA synthetase in extracts from sheep liver is compared to that of lysyl‐tRNA, isoleucyl‐tRNA, leucyl‐tRNA and seryl‐tRNA synthetases by gel filtration on Biogel A‐5m. Extraction conditions are described which lead to isolation of methionyl‐tRNA synthetase exclusively in the form of complexes of molecular weight close to 10 6 . Limited trypsin treatment of these aggregates releases a fully active low‐molecular‐weight form of methionyl‐tRNA synthetase which was purified to a specific activity of 674 units/mg at 25 °C with a yield of 40%. The homogeneous enzyme appears to be undistinguishable from the corresponding enzyme derived from sheep lactating mammary gland, as judged by acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and by titration with antibodies raised against the enzyme purified from liver.