Open AccessReaction Mechanism of Non‐allosteric Phosphofructokinase
Author(s) -
SIMON Wolfgang A.,
HOFER Hans Werner
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12435.x
Subject(s) - allosteric regulation , phosphofructokinase , mechanism (biology) , chemistry , allosteric enzyme , phosphofructokinase 1 , economics , glycolysis , biochemistry , enzyme , physics , quantum mechanics
The reaction mechanism of the non‐allosteric phosphofructokinase from Lactobacillus plantarum was investigated by initial‐rate bisubstrate kinetics and product inhibition kinetics and by the measurement of equilibrium isotope exchange in the presence of various substrate and product concentrations. The reaction mechanism is clearly sequential. The product inhibition and equilibrium isotope‐exchange patterns are consistent with an ordered bi‐bi reaction sequence with fructose 6‐phosphate as the leading substrate and ADP as the first product released from the enzyme.