Open AccessA Proton Nuclear‐Magnetic‐Resonance Study of Self‐Stacking in Purine and Pyrimidine Nucleosides and Nucleotides
Author(s) -
MITCHELL Paul R.,
SIGEL Helmut
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12432.x
Subject(s) - isodesmic reaction , uridine , purine , adenosine , nucleotide , stacking , chemistry , pyrimidine , stereochemistry , crystallography , biochemistry , computational chemistry , enzyme , rna , organic chemistry , density functional theory , gene
The concentration dependence of the chemical shifts of the protons H‐2, H‐8 and H‐1′ of ATP 4− and of Mg(ATP) 2− , of all non‐labile protons of adenosine, of H‐5, H‐6 and H‐1′ of UTP, and of H‐5, H‐6, H‐1′, and H‐2′ of uridine have been measured. The results for the purine derivatives are consistent with the isodesmic model of indefinite non‐cooperative stacking; for adenosine K = 15 ± 2 M −1 , for ATP K = 1.3 ± 0.2 M −1 and for Mg(ATP) 2− K = 3.6 ± 0.3 M −1 . For the pyrimidines, uridine and UTP, stacking is much weaker and the stability constant could only be estimated; for uridine K ⋜ 0.5 M −1 , and for UTP K ≈ 0.3 M −1 .