Open AccessTranscription of Bacteriophage T4 DNA in vitro : Selective Initiation with Dinucleotides
Author(s) -
RÜGER Wolfgang
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12427.x
Subject(s) - transcription (linguistics) , dna , rna polymerase , in vitro , bacteriophage , polymerase , chemistry , microbiology and biotechnology , dna polymerase , biology , rna , biochemistry , gene , escherichia coli , philosophy , linguistics
The transcription products of phage T4 DNA in vitro are separated on polyacrylamide gels. The influence of salt, polymerase, triphosphate concentration and glucosylation on the RNA synthesis are shown. Individual transcripts are initiated selectively with dinucleotides and a single triphosphate. This technique allows the prediction of the initiation sequences of several T4 transcripts.