Open AccessSequence of 129 Nucleotides at the 3′‐Terminus of Encephalomyocarditis Virus RNA
Author(s) -
PORTER Alan G.,
MERREGAERT Josef,
EMMELO John,
FIERS Walter
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12406.x
Subject(s) - microbiology and biotechnology , biology , rna , nucleic acid sequence , oligonucleotide , complementary dna , reverse transcriptase , polymerase , rnase h , primer (cosmetics) , rna dependent rna polymerase , dna , biochemistry , gene , chemistry , organic chemistry
The sequence of 129 nucleotides next to the poly(A)tail of encephalomyocarditis virus RNA has been determined by rapid gel sequencing of cDNA synthesized with DNA polymerase I or reverse transcriptase and a rapid transcriptase and a phaisng primer, [5′‐ 32 P]p(dt) 8 dC. the sequence is in accored with (a) the pyrimideine tracts shich were mapped in blocks along the cDNA, (b) the sequences of seven characteristic T 1 RNase oligonucleotides in the RNA transcribed form the cDNA with RNA polymersase, and (c) a limited amount of sequences deduced by parital spleen phosphodiesterase digestion and depurination of endouclease IV oligonucletodes. The 3′ end shows little secondary strucutre on it own. Ten nonsense condons block all three reading frames such that at least 26 nucleotides do not code for protein. The possible function of a homology A‐A‐U‐A‐A‐A with other poilyadenylated RNAs is discussed.