Open AccessDistribution of AMP‐Deaminase Isozymes in Rat Tissues
Author(s) -
OGASAWARA Nobuaki,
GOTO Haruko,
YAMADA Yasukazu,
WATANABE Tomomasa
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12378.x
Subject(s) - isozyme , biology , biochemistry , kidney , amp deaminase , spleen , microbiology and biotechnology , adenosine deaminase , enzyme , endocrinology , immunology
1 The distribution of AMP deaminase isozymes in rat tissues was analyzed by electrophoresis on cellulose acetate membrane, by chromatography on phosphocellulose column, and by the application of immunological technique empolying specific antisera against three parental AMP deaminases (isozymes A, B and C). Skeletal muscle extracts and diaphragm extracts contain a single identical isozyme, isozyme A. The major isozyme species of liver, kindney and testes are also identical and they are isozyme B. Heart extracts contains isozyme C exclusively. Extracts of brain, lung and spleen contain five isozymes, presumably a complete set of five B–C hybrids. 2 Developmental patterns of AMP deaminase isozyme were studied. In early postnatal life, extracts of heart, liver, kidney ad lung contain five isozymes similar to those observed in adult brain. During postnatal development, a shift to isozyme C occurs in heart, whereas a shift to isozyme B occurs in liver and kidney. Five isozymes in lung remain throught development. In brain a shift ofB to five isozymes is observed during de3velopment. Isozyme A is the predominant form in muscle throught postnatal development. 3 AMP deaminase in the regenerating liver was analyzed, but the data indicated that thre was no change of isozyme distribution during hepatic regeneration.