Open AccessConsequences of a Specific Cleavage in situ of 16‐S Robosomal RNA for Polypeptide Chain Initiation
Author(s) -
BAAN Robert A.,
NAAKTGEBOREN Nico,
CHARLDORP Rein,
KNIPPENBERG Peter H.,
BOSCH Leendert
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12358.x
Subject(s) - ribosome , cleavage (geology) , ribosomal rna , initiation factor , protein biosynthesis , chemistry , protein subunit , stimulation , rna , microbiology and biotechnology , biology , biochemistry , stereochemistry , endocrinology , paleontology , fracture (geology) , gene
The effect of Bacteriocin (cloacin DF13) treatment of Eschdricha coli ribosmes on initiation of protein synthesis has been studied in detail. In agreement with out previous findings [Baan et al. (1976) Proc. Natl. Acad. Sci. U. S. A. 73 , 702–706] it is shown that 70‐S initiation complexes can be formed wiht cloacin‐treated ribosomes, but that the initiation factor IF‐1 does not fuction properly. The following pleiotropic effects of this factor have been studied: (a) the acceleration of ribosomal subunit exchange with 70‐S coples; (b) the stimulation of the IF‐3mediated dissocaiton of 70‐S ribosomes; (c) the stimulation of 30‐S initiation complexes. The effect (a) and (b) are virtually abolished after cleavage of 16‐S rRNA. The effect (d) is only partially reduced wherease effect (c) seems to be unimpaired. It is cocluded that 70‐S initiation complex formation with cloacin‐treated ribosomes suffers from funcitoning of IF‐1 in the generation of active subunits from 70‐S gight coples. This is the only effect on initiaion. It can be compenstaed for by adding more IF‐3. The data privide functional evidence that 16‐S rRNA invloved in ribosomal subunite interaction.