Open AccessAffinity Chromatography of Phenylalanine Hydroxylase
Author(s) -
COTTON Richard G. H.,
JENNINGS Ian G.
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12247.x
Subject(s) - pteridine , phenylalanine hydroxylase , chemistry , sepharose , affinity chromatography , carbodiimide , phenylalanine , enzyme , tetrahydrobiopterin , adsorption , chromatography , stereochemistry , biochemistry , organic chemistry , cofactor , amino acid
1 Four independent methods have established that the structure of a previously reported pteridine affinity adsorbent, 6,7‐dimethyl‐5,6,7,8‐tetrahydropterin–CH‐Sepharose, is 5(CH‐Sepharosyl)‐6,7‐dimethyl‐5,6,7,8‐tetrahydropterin. 2 A novel reaction, the carbodiimide‐promoted coupling of a carboxyl group to N‐5 of a tetrahydropterin, is described. 3 Two novel adsorbents, 5‐formyl‐tetrahydrofolate–AH‐Sepharose and 5‐methyl‐tetrahydrofolate–AH‐Sepharose, are described which may be useful not only in the study of phenylalanine hydroxylase but also in the study of folate‐metabolizing enzymes.