Open AccessFatty Acid β‐Oxidation System in Microbodies of n ‐Alkane‐Grown Candida tropicalis
Author(s) -
KAWAMOTO Susumu,
NOZAKI Chikateru,
TANAKA Atsuo,
FUKUI Saburo
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12130.x
Subject(s) - sodium azide , microbody , catalase , candida tropicalis , hydrogen peroxide , nad+ kinase , chemistry , biochemistry , oxygen , fatty acid , yeast , beta oxidation , enzyme , organic chemistry
Localization of fatty acid β‐oxidation system in microbodies of Candida tropicalis cells growing on n ‐alkanes was studied. Microbodies isolated from the yeast cells showed palmitate‐dependent activities of NAD reduction, acetyl‐CoA formation and oxygen consumption. When sodium azide, an inhibitor of catalase, was added to the system, palmitate‐dependent formation of hydrogen peroxide was observed. Stoichiometric study revealed that two moles of NAD were reduced per one mole of oxygen consumed in the absence of sodium azide and the presence of the inhibitor doubled the oxygen consumption by microbodies without an appreciable change in NAD reduction. These results indicate that the yeast microbodies contain β‐oxidation system of fatty acid, and that catalase located in the organelles participates in the degradation of hydrogen peroxide to be formed at the step of dehydrogenation of acyl‐CoA.