Open AccessConservation of Active Ribosomes in Acetone‐Treated Cells of Tetrahymena pyriformis
Author(s) -
KRISTIANSEN Karsten,
HARTMANN Bolette,
PLESNER Paul,
KRÜGER Anna
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12104.x
Subject(s) - tetrahymena pyriformis , ribosome , tetrahymena , acetone , chemistry , microbiology and biotechnology , biology , biochemistry , rna , gene
The preparation of an acetone powder of cells of Tetrahymena pyriformis GL is described. A comparison of ribosomal particles isolated from acetone‐treated and untreated cells shows that structurally and functionally intact ribosomes can be isolated from acetone‐treated cells. Fully active ribosomes have been isolated from acetone powder of Tetrahymena that had been stored for more than 6 months at 4°C. Thus, this procedure allows easy storage of large quantities of cells for the bulk preparation of active ribosomes.