Open AccessA Naphthoquinone Adsorbent for Affinity Chromatography of Human Dihydropteridine Reductase
Author(s) -
COTTON Richard G. H.,
JENNINGS Ian
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12097.x
Subject(s) - naphthoquinone , tetramer , chemistry , adsorption , chromatography , affinity chromatography , native state , enzyme , reductase , biochemistry , organic chemistry
1. A 1,2‐naphthoquinone adsorbent is described which allows simple purification of dihydropteridine reductase directly from crude extract. 2. The native molecular weight indicates that a tetramer structure of the species is isolated by this method; this is unusual and possibly reflects the capacity of the procedure to preserve the native state of the enzyme.