Open AccessQuantification of Myosin Heavy‐Chain mRNA during Myogenesis
Author(s) -
ROBBINS Jeffrey,
HEYWOOD Stuart M.
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12056.x
Subject(s) - myosin , rna , complementary dna , messenger rna , microbiology and biotechnology , myogenesis , biology , myosin light chain kinase , biochemistry , myocyte , gene
Complementary DNA (cDNA) was synthesized from a 26‐S poly(A)‐containing RNA fraction consisting predominately of myosin mRNA, and used to quantitate the amounts of myosin mRNA during development. RNA · cDNA myosin hybridizations were performed with RNA isolated from tertiary cultures of chick fibroblasts, and the proportion of myosin RNA determined. The levels of myosin RNA present in primary chick myoblast cultures undergoing development were also analyzed by RNA · cDNA myosin hybridizations. Correlation of these values with the rate of myosin heavy chain synthesis indicates that myosin mRNA is subject to post‐transcriptional or translational control.