Open AccessIsolation of Messenger RNA Coding for Eggshell Protein of the DNA‐Eliminating Nematode Ascaris lumbricoides
Author(s) -
ZULAUF Erich,
GUT Charlotte
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12053.x
Subject(s) - rna , polyacrylamide , eggshell , polyacrylamide gel electrophoresis , biology , messenger rna , microbiology and biotechnology , ascaris lumbricoides , complementary dna , gel electrophoresis , biochemistry , gene , helminths , enzyme , ecology , zoology
Poly(A)‐containing RNA from polyploid uterine epithelial cells of Ascaris lumbricoides has been isolated by poly(U)‐Sepharose chromatography. The bulk of poly(A)‐containing RNA migrates as 18‐S RNA in formamide/polyacrylamide gels. In a cell‐free wheat germ system, this RNA directs the synthesis of a polypeptide with identical migration behavior in dodecylsulphate/urea/polyacrylamide gels as the polypeptide isolated from the proteinaceous eggshell. The two proteins reveal almost identical peptide patterns in fingerprint analysis. The authentic eggshell protein has been identified as a glycoprotein with a molecular weight of about 10 000, as determined by dodecylsulphate/polyacrylamide gel electrophoresis. The apparent discrepancy between mRNA length and the required coding length for the protein is discussed.