Open AccessIsolation of 4.5‐Dihydroxyisophthalic Acid, an Inhibitor of Brain Glutamate Decarboxylase, Produced by a Streptomyces Species
Author(s) -
ENDO Akira,
KITAHARA Nobuaki,
OKA Hidehiko,
MIGUCHIFUKAZAWA Yumi,
TERAHARA Akira
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12018.x
Subject(s) - glutamate decarboxylase , streptomyces , biochemistry , metabolite , carboxy lyases , enzyme , glutamate receptor , chemistry , non competitive inhibition , biology , bacteria , receptor , genetics
A potent inhibitor of brain glutamate decarboxylase (L‐glutamate 1‐carboxy‐lyase, EC 4.1.1.15) was isolated from cultures of a Streptomyces species, and its structure was found to be 4,5‐dihydroxyisophthalic acid. The metabolite inhibited beef brain glutamate decarboxylase 50% at a concentration of 0.12 μg/ml (0.61 μM). The kinetic analysis indicated that 4,5‐dihydroxyisophthalic acid was a competitive inhibitor having a K i value of 0.18 μM.