Open AccessPurification and Properties of Phage P22 c 2 Repressor
Author(s) -
BALLIVET Marc,
EISEN Harvey
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12009.x
Subject(s) - repressor , dissociation (chemistry) , mutant , dna , chemistry , operator (biology) , stereochemistry , microbiology and biotechnology , biology , biochemistry , gene , gene expression
The c 2 repressor of phage P22 has been purified to homogeneity. It specifically binds to λimm 21 and P22 DNA. Its affinity for the presumed operator mutant P22 vir B is reduced. The initial dissociation rates of the complex between c 2 repressor and λimm 21 DNA are 0.02 min −1 at 0°C, 0.08 min −1 at 20°C and 0.17 min −1 at 32°C. The dissociation rates of complexes formed between the c 2 repressor and the λ imm 21 operators O R , O L and O R vira were measured and compared to the corresponding rates obtained with 21 c I repressor.