Open AccessStructural Studies on Yeast 3‐Phosphoglycerate Kinase
Author(s) -
FATTOUM Abdellatif,
ROUSTAN Claude,
FEINBERG Jeanne,
DESVAGES Gisèle,
PRADEL LouiseAnne
Publication year - 1978
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1978.tb12007.x
Subject(s) - cyanogen bromide , phosphoglycerate kinase , sephadex , yeast , cleavage (geology) , residue (chemistry) , chemistry , size exclusion chromatography , biochemistry , chromatography , stereochemistry , peptide sequence , biology , enzyme , gene , paleontology , fracture (geology)
Cyanogen bromide cleavage of yeast 3‐phosphoglycerate kinase yielded four fragments which account for the amino acid composition of the entire molecule. These results are consistent with a single polypeptide chain of molecular weight 42000. Affinity chromatography on Sepharosemercurial followed by gel filtration on Sephadex was used with success for separation of peptides. The carboxyl and N‐terminal fragments were characterized. The N‐terminal fragment contained the single cysteinly residue of the protein. After cyanylation and subsequent cleavage, this cysteinyl residue was located near position 100.