Open AccessHydrodynamic Properties of 2‐Mercaptoethanol‐Modified Glycogen
Author(s) -
GEDDES Robert,
HARVEY John D.,
WILLS Peter R.
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11971.x
Subject(s) - glycogen , iodoacetamide , chemistry , glycogen branching enzyme , diffusion , particle (ecology) , extraction (chemistry) , chromatography , glycogen phosphorylase , biochemistry , cysteine , biology , thermodynamics , enzyme , ecology , physics
Treatment of glycogen with 2‐mercaptoethanol and iodoacetamide gives rise to a modified glycogen which resembles the original glycogen in its hydrodynamic behaviour but has a pronounced tendency to aggregate. The modified glycogen can be distinguished easily, by its diffusion coefficient, from glycogen degraded by more traditional methods of extraction. The ‘fundamental’ glycogen particle appears to be composed of two or three glycogen β‐particles linked by a single protein chain.