Open AccessLipopolysaccharides de mutants thermosensibles de Escherichia coli K12
Author(s) -
BRUNETEAU Maud,
MICHEL Georges
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11939.x
Subject(s) - mutant , escherichia coli , lipopolysaccharide , chemistry , strain (injury) , biochemistry , microbiology and biotechnology , biology , gene , anatomy , endocrinology
Lipopolysaccharides from Thermosensitive Mutants of Escherichia coli K12 Thermosensitive mutants of Escherichia coli K12 were grown at 30°C and 40°C. The serologic properties and the composition of their lipopolysaccharides were investigated. The inhibition of hemagglutination by the lipopolysaccharides of various mutant strains was tested against the anti‐ E. coli K12 CR34 system. An inhibition was observed with all the mutants but one, CR 34 T83 which had no inhibitory effect. Chemical analysis of lipopolysaccharides and mass spectrometric analysis of their methylated derivatives indicated the presence of the same components in the various lipopolysaccharides: glucose, galactopyranose, galactofuranose, heptopyranose and heptofuranose. However the 2,3,4 tri‐ O ‐methyl glucose is missing in the lipopolysaccharide of the mutant T83. This result agrees with the absence of a substituent on the 6‐position of the non‐reducing core‐terminal glucose. The lipopolysaccharide of the T83 mutant has the complete core‐type of E. coli K12. The relations of mutations with modifications of the composition of inner and outer envelopes in various mutants are discussed.