Open AccessSugar‐Specific Antibodies Reactive towards Cell‐Surface Carbohydrates
Author(s) -
BLOCH Robert,
MACCECCHINI Maria Luisa,
JUMBLATT James,
BUTTRICK Peter,
BURGER Max M.
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11878.x
Subject(s) - monosaccharide , concanavalin a , sugar , chemistry , affinity chromatography , biochemistry , antibody , cell , elution , chromatography , biology , enzyme , in vitro , immunology
Antibodies generated against sugar‐azoproteins have been purified by affinity chromatography using elution with monosaccharide. They have high specificity for monosaccharide and they react with terminal, non‐reducing sugars on the surfaces of human erythrocytes. Some of them, either native or as Fab fragments, inhibit mitogenic stimulation of lymphocytes by concanavalin A.