Open AccessOn the Distribution and Packing of RNA and Protein in Ribosomes
Author(s) -
SERDYUK Igor N.,
GRENADER Arkadiy K.
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11833.x
Subject(s) - ribosome , rna , gyration , scattering , ribosomal rna , crystallography , chemistry , ribosomal protein , physics , molecular physics , biochemistry , optics , gene , mathematics , geometry
From the analysis of the measured radii of gyration of the RNA ( R g = 6.6 ± 0.3 nm) and protein ( R g = 10.2 ⊥ 0.5 nm) components of the 50‐S subparticle of Escherichia coli ribosomes it is concluded that proteins containing a large amount of hydrodynamically bound water are located on the periphery of the tightly packed RNA. We found that the common features of the measured X‐ray scattering curves of the E. coli 70‐S ribosome, its 30‐S and 50‐S subparticles and wheat 80‐S ribosomes in the region of scattering angles corresponding to scattering vectors μ from 1 to 5 nm ‐1 reflect features of the RNA compact packing. A hypothesis is proposed that the compact packing of RNA helices in the range of Bragg distances of 4.5–2.0 nm is a general structural feature of all ribosomal particles.