Open AccessStudies on the Transcription Complex of Escherichia coli RNA Polymerase
Author(s) -
ROHRER Hermann,
ZILLIG Wolfram
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11822.x
Subject(s) - microbiology and biotechnology , polymerase , rnase p , dna polymerase i , transcription bubble , rnase h , biology , rna polymerase , dna , dna clamp , nuclease , transcription (linguistics) , primase , dna polymerase ii , biochemistry , rna polymerase i , ternary complex , coding strand , rna , rna dependent rna polymerase , reverse transcriptase , enzyme , gene , linguistics , philosophy
To study the chain elongation phase of enzymatic RNA synthesis ternary transcription complexes with T7 DNA or poly[(dA) · (dT)] as template were isolated by gel exclusion chromatography. The DNA in these complexes contains single‐stranded regions which are recognized by a single‐strand‐specific nuclease from Neurospora crassa. The non‐codogenic DNA strand in the poly[(dA) · (dT)] ternary complex is preferentially hydrolysed by the nuclease. The polymerase protects predominantly the codogenic strand in this complex from digestion by DNAse I. In the T7 DNA ternary complex a DNA fragment with a chain length of approximately 26 nucleotides and an RNA fragment of about 22 nucleotides are protected by polymerase from digestion by DNAse and RNAse.