Open AccessCell‐Wall Lipopolysaccharide of the ‘ Shigella ‐like’ Escherichia coli 058
Author(s) -
DMITRIEV Boris A.,
KNIREL Yuriy A.,
KOCHETKOV Nikolay K.,
JANN Barbara,
JANN Klaus
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11789.x
Subject(s) - chemistry , polysaccharide , mannose , tetrasaccharide , rhamnose , chromatography , biochemistry
Two lipopolysaccharide preparations were obtained from Escherichia coli 058 by extraction with 45% aqueous phenol and fractional precipitation with cetyltrimethyl ammonium bromide (Cetavlon). Chemical anylysis and polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate showed that the two preparations differed only in the extent of the O‐specific polysaccharide moiety. The O‐specific polysaccharide was characterized with proton magnetic resonance and infrared spectroscopy, optical rotation and paper electrophoresis. Using gas‐liquid chromatography and ion‐exchange chromatography, it was shown to contain D‐mannose, 2‐acetamido‐2‐deoxy‐D‐glucose, 3‐ O ‐(‐ R ‐1′‐carboxyethyl)‐L‐rhamnose (rhamnolactylic acid). and O ‐acetyl groups in the molar ratios of 2: 1: 1: 1. The polysaccharide and oligosaccharides obtained from it were subjected to methylation and chromic acid oxidation. The results obtained indicated that the polysaccharide consists of tetrasaccharide repeating units in which the trisaccharide β‐GlcNAcl – 4αMan‐1–4(2/3‐ O ‐Ac)‐Man is substituted at C‐3 of the non‐acetylated mannose with rhamnolactylic acid. The repeating units are joined through α‐mannosyl‐1–3‐glucosamine bonds. This structure is identical with that of the cell wall polysaccharide of Shigella dysenteriae type 5.