Open AccessIdentification of 2‐Methyl‐3‐hydroxydecanoic and 2‐Methyl‐3‐hydroxytetradecanoic Acids in the ‘Lipid X’ Fraction of the Bordetella pertussis Endotoxin
Author(s) -
HAEFFNER Nicole,
CHABY Richard,
SZABÓ Ladislas
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11696.x
Subject(s) - bordetella pertussis , trifluoroacetic acid , fraction (chemistry) , chemistry , mass spectrometry , chromatography , organic chemistry , biology , bacteria , genetics
The ‘lipid X’ fraction, released from the Bordetella pertussis endotoxin upon treatment with trifluoroacetic acid of pH 3 at 50°C, was shown to contain, in addition to 3‐hydroxydecanoic, 3‐hydroxydodecanoic, 3‐hydroxytetradecanoic, and tetradec‐2‐enoic acids, 2‐methyl‐3‐hydroxydecanoic‐, and 2‐methyl‐3‐hydroxytetradecanoic acids. The structure of these was established by gasliquid chromatography/mass spectrometry.