Open AccessDimerization of Papain Induced by Mercuric Chloride and a Bifunctional Organic Mercurial
Author(s) -
SLUYTERMAN Lamoraal A. AE.,
WIJDENES Jacob,
VOORN Gysbertus
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11647.x
Subject(s) - bifunctional , papain , chemistry , reagent , chloride , titration , thiol , size exclusion chromatography , molecule , inorganic chemistry , organic chemistry , catalysis , enzyme
The bifunctional mercurial meso ‐1,4‐bis(acetatomercuri)‐2,3‐diethoxybutane and mercuric chloride are capable of dimerizing papain, by the attachment of the thiol group of two molecules of papain to each molecule of reagent. This is evident from the titration data, gel filtration and sedimentation equilibrium. The conformational change of papain necessary for this reaction is discussed.