Open AccessWater‐Soluble Glycosphingolipids of Dog Gastric Mucosa
Author(s) -
SLOMIANY Amalia,
SLOMIANY Bronislaw L.
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11619.x
Subject(s) - glycolipid , ceramide , chemistry , hydrolysis , galactose , enzyme , biochemistry , hapten , tetrahydrofuran , enzymatic hydrolysis , chromatography , stereochemistry , antigen , biology , apoptosis , solvent , genetics
Two glycosphingolipids, the carbohydrate portion of which consisted of galactose, glucose and N ‐acetylgalactosamine, have been isolated from the aqueous phase of buffered tetrahydrofuran extract of dog gastric mucosa. The structures of these glycolipids were identified by partial acid hydrolysis, sequential degradation with specific glycosidases and methylation analysis. The structure of glycolipid I (GalNAcα1→3GalNAcβ1→3Galα1→4Galβ1→4Glc→ceramide) was found to be identical to that of Forssman hapten. The branched structure of glycolipid II, as determined by the combination of enzymatic degradation and comparison of the permethylation products of the enzyme‐degraded compounds, is proposed to be: