Open AccessSomatic Antigens of Shigella
Author(s) -
DMITRIEV Boris A.,
KNIREL Yuriy A.,
KOCHETKOV Nikolay K.,
HOFMAN Irihna L.,
ČAPEK Karel
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11612.x
Subject(s) - polysaccharide , shigella dysenteriae , lipopolysaccharide , biochemistry , shigella sonnei , glycan , chemistry , microbiology and biotechnology , galactose , hydrolysis , acid hydrolysis , biology , shigella , escherichia coli , glycoprotein , gene , endocrinology
The O‐specific polysaccharide obtained from Shigella dysenteriae type‐2 lipopolysaccharide by mild acid hydrolysis consisted of N ‐acetylgalactosamine, N ‐acetylglucosamine, d ‐galactose, d ‐glucose, and O ‐acetyl group in the ratio of 2:1:1:1:1. A number of oligosaccharides were obtained by deamination of the N ‐deacetylated polysaccharide and by Smith degradation of the both native and O ‐deacetylated polysaccharides. The identification of oligosaccharides along with methylation analysis and chromic anhydride oxidation showed that the polysaccharide was built up of the repeating pentasaccharide units whose proposed structure is given below: