Open AccessEnergy‐Dependent Transport of Manganese into Yeast Cells and Distribution of Accumulated Ions
Author(s) -
OKOROKOV Lev. A.,
LICHKO Lidiya P.,
KADOMTSEVA Valentina M.,
KHOLODENKO Vasiliy P.,
TITOVSKY Victor T.,
KULAEV Igor S.
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11538.x
Subject(s) - manganese , vacuole , divalent , cytosol , sodium azide , yeast , chemistry , biophysics , cyanide , biochemistry , glucose transporter , protoplast , dinitrophenol , azide , antimycin a , sodium cyanide , ion transporter , electron transport chain , inorganic chemistry , enzyme , membrane , biology , organic chemistry , cytoplasm , insulin , endocrinology
Manganese transport into yeast cells is energy‐dependent. It is dependent on endogenous sources of energy and is inhibited by olygomycin (12.5–25 μg/ml), 2,4‐dinitrophenol (1 mM), 2‐deoxyglucose (1–50 mM) and sodium azide (1–10 mM), but is stimulated by cyanide and glucose. The stimulating effect of glucose is eliminated by N ‐ethylmaleimide and iodoacetate, which apparently inhibit the transport of glucose itself. About 75% of the manganese accumulated in the presence of glucose is found in yeast protoplasts and nearly 25% in the cell walls. A major portion of the accumulated manganese is found in vacuoles. The concentration of osmotically free manganese in the cytosol did not exceed 2 mM, but the concentration in vacuoles was up to 14 mM. The tonoplast is assumed to have a transport system for divalent cations, thereby regulating their concentration in the cytosol.