Open AccessThe Nature of DNA Synthesized in Nuclei from Mouse L1210 Cells
Author(s) -
MÜLLBACHER Arno,
RALPH Ray K.
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11535.x
Subject(s) - l1210 cells , dna , in vitro , dna synthesis , microbiology and biotechnology , biology , chemistry , biochemistry , cytotoxicity
A DNA‐synthesizing system in vitro from mouse L1210 cell nuclei is described. A high proportion of the DNA synthesized by the L1210 cell nuclei in vitro appeared to be covalently linked complementary DNA. DNA synthesis by L1210 nuclei was not inhibited by the anti‐leukaemia compound 4′‐(9‐acridinylamino)‐methansulphon‐ m ‐anisidine, although this drug binds to isolated DNA and is active against L1210 leukaemia.