Open AccessSmall‐Angle X‐Ray Studies of the Human Immunoglobulin Molecule Kol
Author(s) -
PILZ Ingrid,
SCHWARZ Erika,
PALM Walter
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11517.x
Subject(s) - antibody , molecule , x ray , chemistry , materials science , physics , biology , optics , immunology , organic chemistry
The conformation of the human immunoglobulin molecule Kol [IgG I, χ 2 γ 2 , Gm(f) + ] was studied by small‐angle X‐ray scattering in 0.15 M NaCl solution. The radius of gyration was found to be 5.84 ± 0.04 nm, the volume 329 ± 15 nm 3 and the molecular weight 150000 ± 10000. Information on the overall shape was obtained by comparing the experimental scattering curve with the calculated curves for various models. The models were obtained by arranging the models found for the Fab and Fc fragments of the same immunoglobulin molecule in a different manner. A model which fits all the date and the form of the experimental scattering curve is presented.