Open AccessLeast‐Squares Analysis of Circular Dichroic Spectra of Proteins
Author(s) -
HAMMONDS R. Glenn
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11407.x
Subject(s) - myoglobin , dichroic glass , lysozyme , chemistry , papain , spectral line , analytical chemistry (journal) , least squares function approximation , crystallography , mathematics , chromatography , physics , enzyme , optics , biochemistry , statistics , astronomy , estimator
It is shown that the method proposed by Baker and Isenberg [ Biochemistry , 15 , 629 (1976)] for estimating secondary structure composition of proteins from circular dichroic spectra is a leastsquares fitting technique. Estimates obtained by this method for myoglobin, lysozyme, lactate dehydrogenase, papain, and ribonuclease are not substantively different from those obtained using unconstrained linear least squares.