Open AccessCharacterization of Foldback Sequences in Physarum polycephalum Nuclear DNA Using the Electron Microscope
Author(s) -
HARDMAN Norman,
JACK Peter L.
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11391.x
Subject(s) - physarum polycephalum , dna , checkerboard , mathematics , biology , genetics , geometry , microbiology and biotechnology
An examination of the foldback fraction of nuclear DNA from Physarum polycephalum has been carried out using the electron microscope. Results show that the inverted repeat sequences responsible for the formation of foldback DNA range from 150–3000 bases in length, with a number‐average size of 340 bases. About one‐half of the inverted sequences form looped structures with loop sizes averaging 1200 bases in length. The distance between adjacent foldback sequences is estimated to be in the range 100–1500 bases.