Open AccessRNA Sequences in Ribonucleoprotein Fragments of the Complex Formed from Ribosomal 23‐S RNA and Ribosomal Protein L24 of Escherichia coli
Author(s) -
BRANLANT Christiane,
SRI WIDADA Johanes,
KROL Alain,
EBEL JeanPierre
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11377.x
Subject(s) - rna , 5.8s ribosomal rna , ribosomal rna , ribosomal protein , ribonucleoprotein , microbiology and biotechnology , ribosome , ribonuclease , 5s ribosomal rna , biology , 50s , chemistry , biochemistry , gene
Upon digestion of the complex formed from the 23‐S ribosomal RNA and the 50‐S ribosomal protein L24 of Escherichia coli , two fragments resistant to ribonuclease were recovered; these fragments contained RNA sections belonging to the 480 nucleotides at the 5′ end of 23‐S RNA. By determining the sequence of 70% of this latter region we were able to localise the sections which, in the presence of the protein, are resistant to ribonuclease. Our results suggest that the region encompassing the 480 nucleotides starting at the 9th nucleotide from the 5′ end of 23‐S RNA has a compact tertiary structure, which is stabilised by protein L24.