Open AccessProstaglandin E 2 Production in 3T3 Cells Transformed by Polyoma Virus Raises the Intracellular Adenosine 3′: 5′‐Monophosphate Levels
Author(s) -
CLAESSON HansErik,
LINDGREN Jan Åke,
HAMMARSTRÖM Sven
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11361.x
Subject(s) - prostaglandin e2 , adenosine , intracellular , prostaglandin , prostaglandin e , chemistry , endocrinology , endogeny , cyclic adenosine monophosphate , medicine , fibroblast , biology , biochemistry , in vitro , receptor
High cellular adenosine 3′: 5′‐monophosphate (cyclic AMP) levels were found in a polyoma‐virus‐transformed 3T3 fibroblast line, which produces comparatively high prostaglandin E 2 concentrations. Prostaglandin E 2 and cyclic AMP increased with time during 6 days of growth. Both effects were prevented by three different cyclo‐oxygenase inhibitors. Addition of prostaglandin E 2 , at a concentration which would have been synthesized in the absence of inhibitor, reversed the effect of indomethacin, one of the cyclo‐oxygenase inhibitors, on cyclic AMP. It is concluded that endogenous prostaglandin E 2 production in these transformed cells influences their cellular cyclic AMP levels.