Open AccessNucleotide Sequence of a Region in 23‐S RNA Adjacent to Peptidyl Transferase Catalytic Center of Escherichia coli Ribosomes
Author(s) -
YUKIOKA Munehiko,
HATAYAMA Takumi,
OMORI Koichiro
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11337.x
Subject(s) - peptidyl transferase , ribosome , escherichia coli , nucleotide , nucleic acid sequence , rna , genetics , microbiology and biotechnology , sequence (biology) , transferase , chemistry , biology , biochemistry , gene , enzyme
N ‐Iodoacetylphenylalanyl‐tRNA Phe was used as an affinity label to localize the RNA components intimately involved in the catalytic center of Escherichia coli ribosomes. This analogue could alkylate the specific region of 23‐S RNA that was located within 2000 nucleotides from the 3′ terminus of the molecule. Sequence analysis revealed that the alkylation by the active substrate ( N ‐iodo‐acetylphenylalanyl‐tRNA Phe ) was directed to the 5′‐terminal adenosine residue of a heptanucleotide, A‐U‐U‐U‐U‐A‐Gp, which seemed to be derived from a heptadecanucleotide, U‐U‐A‐A‐A‐A‐A‐C‐A‐C‐A‐U‐U‐U‐U‐A‐Gp, in the original 23‐S RNA. The significance of the unique sequence in the ribosomal functions is discussed.