Open AccessEntry of mRNA into Polyribosomes during Recovery from Starvation in Mouse Sarcoma 180 Cells
Author(s) -
SONENSHEIN Gail E.,
BRAWERMAN George
Publication year - 1977
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1977.tb11320.x
Subject(s) - polysome , ribosome , nucleoprotein , messenger rna , protein biosynthesis , biology , translation (biology) , starvation , microbiology and biotechnology , biochemistry , rna , gene , endocrinology
Starvation in mouse sarcoma 180 cells leads to a block in initiation of protein synthesis which results in the conversion of polysomes to 80‐S ribosomes. The mRNA is released as nucleoprotein particles. Addition of nutrients to the starved cell causes the rapid uptake of the mRNA into large polysomal structures. The ribosomes enter these structures more gradually, without much effect on the sedimentation profile of the labeled mRNA. The results indicate that the stored mRNA in starved cells is readily available for uptake into polysomes. They also suggest that the mRNA may be binding to components other than ribosomes during the initial stages of polysome formation.