Open AccessEsterolytic Activities of Rat Intestinal Mucosa
Author(s) -
NÉGREL Raymond,
SERRERO Ginette,
AILHAUD Gérard,
FERNANDEZLOPEZ Victor
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb11111.x
Subject(s) - hydrolase , endoplasmic reticulum , lipase , chemistry , glycerol , enzyme , hydrolysis , biochemistry , intestinal mucosa , colipase , hydrolytic degradation , triacylglycerol lipase , medicine
The preferential cellular distribution in the villus tip and the subcellular localization in the endoplasmic reticulum of an intestinal glycerol‐ester hydrolase from rat mucosa are described. The enzyme is shown not to be from either pancreatic or bacterial origin; it catalyzes the hydrolysis of short‐ and medium chain triglycerides and of ρ‐nitrophenylacetate. Contrarily to the specificity found for the pig intestinal lipase (Serrero, Négrel and Ailhaud, 1975), no activity is detectable against acyl‐CoA; a thiolester hydrolase different from the glycerol‐ester hydrolase was demonstrated after differential solubilization and chromatographic separation. A high proportion of glycerol‐ester hydrolase is present in the intestinal lumen; its possible complementary role in lipid degradation is discussed.