Open AccessThe Translation in vitro of mRNA from Developing Cysts of Artemia salina
Author(s) -
GROSFELD Haim,
LITTAUER Uriel Z.
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb11050.x
Subject(s) - brine shrimp , artemia salina , messenger rna , biology , in vitro , translation (biology) , protein biosynthesis , embryo , larva , microbiology and biotechnology , biochemistry , anatomy , gene , botany , chemistry , fishery , organic chemistry , toxicity
Succcssive stages in the development of the brine shrimp cyst were used as a model for studying differentiation at the level of mRNA transcription and translation. The poly(A)‐containing mRNA from dormant cysts and free‐swimming larvae (nauplii) was found to be efficiently translated in a Wheat‐germ cell‐free system, and electrophoretic patterns of translation products in vitro resembled those of the endogenous proteins extracted from the equivalent developmental stages. Each stage, however, exhibits a characteristic protein pattern. Two low‐molecular‐weight proteins prominent in the cyst disappeared almost completely in the nauplius stage, whereas the proportion of actin increased 3‐fold. Parallel patterns were observed upon translation in vitro of the respective mRNA preparations. The percentage of the acidic protein, tubulin, decreased somewhat during development.