Open AccessOn the Initiation of Mammalian RNA Polymerase at Single‐Strand Breaks in DNA
Author(s) -
DREYER Christine,
HAUSEN Peter
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10956.x
Subject(s) - polymerase , primer (cosmetics) , microbiology and biotechnology , dna polymerase , chromatin , chemistry , dna , dna clamp , primase , rna , transcription bubble , biology , rna dependent rna polymerase , biochemistry , gene , reverse transcriptase , organic chemistry
Mammalian RNA polymerase B is able to initiate at single‐strand breaks in the DNA template. A 3′‐OH end at a nick is required for initiation whereas the 5′‐end may be either ‐OH or phosphate. The 3′‐OH group does not function as a primer. An appreciable part of the newly synthesized RNA started at a nick remains associated with the DNA in hydrid form. Initiation of exogeneous RNA polymerase B on chromatin exhibits similar requirements.