Open AccessThe Immediate Uptake of Potassium Ion by Mitochondria Requiring Gramicidin and 2,2‐Dimethylsuccinic Anhydride
Author(s) -
HADLER Herbert I.,
DEMETRIOU John M.
Publication year - 1976
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1976.tb10888.x
Subject(s) - chemistry , valinomycin , gramicidin , potassium , succinic anhydride , ion , rotenone , antimycin a , inorganic chemistry , rubidium , mitochondrion , membrane , organic chemistry , biochemistry
The addition of 2,2‐dimethylsuccinic anhydride to mitochondrial suspensions fortified with gramicidin and potassium ion but without any permeant anion caused an immediate and rapid increase in volume (as indicated by absorbance change at 520 nm) and the uptake of potassium ion (as indicated by a cation‐specific electrode). The phenomena was not inhibited by rutamycin but was inhibited by either rotenone, antimycin or 2,4‐dinitrophenol. Rotenone inhibition was relieved by succinate thus orte of the requirements of the process was energy derived from endogenous substrates. Potassium ion Gould be replaced by rubidium and cesium ions but not by lithium or sodium ions. Since 2,2dimethylsuccinate could not replace the anhydride and was not a permeant anion there must also be a requirement for the anhydride bond. The action of the anhydride an the mitochondria must be direct. Only closely related anhydrides were capable of engendering the effect of a highly effective permeant anion